Pharmacological Study of a Nanoencapsulated System and Histopathological Analysis

Abstract

This study is part of the promotion of innovative approaches in wound healing and nanomedicine. It focuses on the synthesis, formulation, and evaluation of a transdermal silver nanoparticles-based patches, designed to accelerate tissue regeneration while minimizing infection risk. Silver nanoparticles (AgNPs) were synthesized using a solvent-free mechanochemical technique and incorporated into a polymer-based biofilm patch. The synthesized nanoparticles were characterized by UV-vis spectroscopy, dynamic light scattering (DLS), and zeta potential analysis, confirming particle formation with adequate stability and size distribution. An ex vivo permeability study using Franz diffusion cell demonstrated sustained AgNPs release through excised mouse skin, validating its ability to penetrate the cutaneous barrier. In in vivo tests, full-thickness wounds were induced in Wistar rats and treated with the AgNPs-based patches. Compared to control and commercial reference treatment (Cicatryl®), the AgNPs-treated group exhibited accelerated healing with over 89% wound contraction by day 5, and complete closure by day 21. Histological analyses confirmed early re-epithelialization, organized collagen matrix, and reappearance of skin appendages in the AgNPs-treated group, suggesting enhanced regenerative activity. These findings confirm the regenerative potential of AgNPs-based systems and highlight their relevance in modern pharmacological wound management

This study is part of the promotion of innovative approaches in wound healing and nanomedicine. It focuses on the synthesis, formulation, and evaluation of a transdermal silver nanoparticles-based patches, designed to accelerate tissue regeneration while minimizing infection risk. Silver nanoparticles (AgNPs) were synthesized using a solvent-free mechanochemical technique and incorporated into a polymer-based biofilm patch. The synthesized nanoparticles were characterized by UV-vis spectroscopy, dynamic light scattering (DLS), and zeta potential analysis, confirming particle formation with adequate stability and size distribution. An ex vivo permeability study using Franz diffusion cell demonstrated sustained AgNPs release through excised mouse skin, validating its ability to penetrate the cutaneous barrier. In in vivo tests, full-thickness wounds were induced in Wistar rats and treated with the AgNPs-based patches. Compared to control and commercial reference treatment (Cicatryl®), the AgNPs-treated group exhibited accelerated healing with over 89% wound contraction by day 5, and complete closure by day 21. Histological analyses confirmed early re-epithelialization, organized collagen matrix, and reappearance of skin appendages in the AgNPs-treated group, suggesting enhanced regenerative activity. These findings confirm the regenerative potential of AgNPs-based systems and highlight their relevance in modern pharmacological wound management

This study is part of the promotion of innovative approaches in wound healing and nanomedicine. It focuses on the synthesis, formulation, and evaluation of a transdermal silver nanoparticles-based patches, designed to accelerate tissue regeneration while minimizing infection risk. Silver nanoparticles (AgNPs) were synthesized using a solvent-free mechanochemical technique and incorporated into a polymer-based biofilm patch. The synthesized nanoparticles were characterized by UV-vis spectroscopy, dynamic light scattering (DLS), and zeta potential analysis, confirming particle formation with adequate stability and size distribution. An ex vivo permeability study using Franz diffusion cell demonstrated sustained AgNPs release through excised mouse skin, validating its ability to penetrate the cutaneous barrier. In in vivo tests, full-thickness wounds were induced in Wistar rats and treated with the AgNPs-based patches. Compared to control and commercial reference treatment (Cicatryl®), the AgNPs-treated group exhibited accelerated healing with over 89% wound contraction by day 5, and complete closure by day 21. Histological analyses confirmed early re-epithelialization, organized collagen matrix, and reappearance of skin appendages in the AgNPs-treated group, suggesting enhanced regenerative activity. These findings confirm the regenerative potential of AgNPs-based systems and highlight their relevance in modern pharmacological wound management