Assessment of The Techniques of The Sperm Preparation of Bovine

Abstract

The evaluation of the quality of the sperm is indispensablefor its usefulness in artificial insemination. For this reason, there are several tests and analyses of the sample using different methods in order to guarantee a sperm of good quality. In this study, we have studied the semen of bulls using different techniques, the light microscope, CASA and the flow cytometry, which determines parameters that allow us to evaluate the quality of bovine sperm. For this purpose, volume, concentration, motility, activity, pH and morphology were evaluated using the "light microscope" technique. On the other hand, viability was assessed by CASA and flow cytometry, which is one of the most elaborate techniques. We then conducted a comparative study between these previous methods in order to determine the reliability and efficiency of each one.